DEAD IN SHELL YOUNGSTERS AVIARY

By Dr   BSc, BVSc, MRCVS, MACVSc (Avian health)

I regularly receive calls from aviculturists that fertile eggs are failing to hatch i.e. that the embryos these eggs contain are dying in the incubation period. Many aviculturists immediately think of Salmonella when they see this, when in fact all infections together including Salmonella account for less than 5% of all dead in-shell youngsters. So just why do these youngsters die? Most young that die in the egg usually die either in the first few days of incubation, or alternatively the last few days of incubation.

In the first few days, embryo death is usually due to either inadequate incubation leading to too low a temperature to keep the chick alive, excessive jarring of the egg that either fatally damages the chick or yolk, or alternatively, a genetic problem affecting the chick which is incompatible with life.

Towards the end of incubation, chicks usually die as a result of problems associated with hatching. As incubation ends; the chick has to shift from getting its oxygen through the membranes that surround it, to breathing air. It also re-absorbs its yolk sac which supplies it with both food and immunity. If the temperature or humidity is incorrect at this time these processes fail to occur correctly and the chick can die.

Between the beginning and end of incubation, the chick is essentially just growing and it is here that nutrition and infection become more important. If the young chick is lacking a nutrient it needs for growth or becomes infected; it may die.

This past year has been a particularly good breeding season for me. Nearly every egg that was fertile has hatched and the resultant youngster has been successfully weaned. Although pleasing, this situation is not always the case despite the best of care. I did however, have one aviculturist mention to me last week that he had had 30% of all fertile eggs fail to hatch. He did not seen overly concerned and appeared to think that nothing could be done. This is far from the truth. An embryo fatality of 5% could be regarded as normal. Anything more than this should arouse suspicions of a problem.

For those of you having a problem with dead-in-the-shell youngsters, lets have a look at the potential problems that can arise with each of these periods of incubation in more detail, so that hopefully the problem can be solved.

Embryonic Death At The Start Of Incubation
Deaths early in incubation can be detected by opening the egg and seeing that it is in fact fertile, but that the embryo is only poorly developed. As mentioned earlier, the usual cause is poor incubation leading to the egg becoming cold after development has started. Possible causes include improper nesting material, over interference by the aviculturist, inadequate control of nest mites, overcrowding in the aviary, old arthritic birds, poor nest box design, competition with other birds within the aviary, poor parenting, nest box too hot or too cold or poorly ventilated, outside disturbances etc. Also as mentioned earlier, eggs are very vulnerable to vibration type injuries early in incubation. Shaking or jarring can kill the developing embryo either directly or by rupturing the yolk. This is of particular relevance when eggs are being transferred for fostering. Embryos that are unlucky enough to have genetic abnormalities usually also die early in incubation. Genetic problems are more likely to occur with in-breeding.

Deaths From Day 4 To Day 14 Of Incubation
This is the longest period of the incubation process and yet it is the time when least deaths occur. The embryo is simply growing. The growing chick receives its nutrition from the yolk and deaths here can reflect nutritional problems in the hen. Hens that are correctly fed are more likely to produce nutritious yolks that support healthy embryos. The effect of breeding bird nutrition is very underrated. By simply feeding a blend of 2-3 seeds and a calcium supplement such as grit, it is not possible to prepare the hens for breeding. Aviculturists who believe they can do this often accept as normal an elevated embryo death rate or several weak chicks in the nest.

Although embryos can die of infection at any time during incubation, it is at this time of growth that they are most vulnerable. Certainly, there are some infections that can be carried by the hen that can infect the ovary such as Chlamydia and Salmonella. These can be incorporated into the egg at the time of its formation, and subsequently infect and kill the embryo as it grows. Infection can also pass through the oviduct wall into the egg. However, these types of infections, that enter the egg prior to laying, are in the minority. Most infections that develop are caught in the nest after hatching. Nests that are dirty, poorly ventilated or excessively humid lead to egg- shell contamination and movement of infectious agents into the egg. Egg quality is also important. Cracked, thin, mis-shapen or rough eggs allow easier entry of infection and are more subject to trauma. Poor eggs can be due to oviduct disease, but are more often associated with a nutritional deficiency: in particular calcium. Some aviculturists will have noticed eggs with translucent clear lines running around the outside of the egg showing the eggs rotations, as it was passing down the oviduct. These thin areas can be an early sign of calcium deficiency.

Embryonic Deaths At The End Of Incubation
Through incubation a membrane called the chorioallantois develops around the chick. The chorioallantois acts a bit like a human placenta, in that it delivers air to the embryo after it diffuses through the shell. At the end of incubation the chick must swap from a chorioallantoic respiration to breathing air. It does this in two stages. First it internally pips. This involves cutting a small hole into the air chamber at the end of the egg and starting to breath the air it contains. At this stage vibrations can be felt in the egg and chick will sometimes vocalize.

After another 12-36 hours the second stage begins, with the chick cracking the shell and breathing external air. While this is happening the last of the yolk sac which is the chicks nutrition during incubation, is drawn into the navel. This eventually ends up as a tiny sac in the wall of the small intestine called Merkels diverticulum and lasts the whole life of the bird. Interestingly, during this time, the chick also drinks the clear fluid around it called the amniotic fluid. This amniotic fluid, and also the yolk sac contain the antibodies that protect the chick from infection in the first few weeks of life.

While all this complex physiology is going on the chick is vulnerable to problems. Too high or low temperature or humidity during this time will adversely affect the chick. The usual problem is too high a temperature, or too low a humidity. This combination causes the shell and shell membrane to become hard and dry. This can lead to a healthy chick becoming exhausted. In addition to this, the chick quickly becomes dehydrated. I am sure many of you, myself included, have helped these chicks hatch only to find them dead later. These chicks often die because they are dehydrated. Such chicks if given small drops of water will often suck them down greedily and survive. These dehydrated chicks are called “sticky chicks” because of the way they stick to the dry shell membranes. They are often found dead after hatching ¼ to1/2 the way, emerging from the shell. If removed from the shell they often have unabsorbed yolk sacs and there is often dry, gluggy albumen still in the egg.

For consistently high hatch rates, it is vital the breeding birds have access to either rain or a bath around this time. If not possible the underside of the hen and also the eggs can be lightly misted with water from a spray bottle. In most species, ideally the nest box should have a humidity of 70% and the sitting bird needs to keep the eggs at between 36.5 to 37.0 degrees celcius. If unsure, a thermometer and hygrometer can be placed in the nest box.

In summary, hatchability can be dramatically improved by three simple steps:

Improving nutrition in the months prior to breeding.
A clean nest for every round, and ongoing nest box hygiene.
Access to rain or a bath around hatching.

If attending to these matters does not help, your avian veterinarian will usually want to review the aviary environment and your management practises, test the hen for infection, or do an egg autopsy.